50X TAE

CAT No. #77607

Description
GBP 50X TAE buffer is a commonly used buffer solution in gel electrophoresis for DNA and RNA analysis. TAE stands for Tris-Acetate-EDTA, representing the three key components of the buffer. Tris, a weak base, helps maintain a stable pH during the electrophoresis process. Acetate ions provide conductivity to the buffer, allowing for efficient current flow. EDTA, a chelating agent, helps protect the DNA or RNA by sequestering divalent cations that can degrade nucleic acids. 50x TAE buffer is used as both the running buffer and the gel matrix preparation buffer. It helps create an electrically conductive environment for the movement of charged DNA or RNA molecules during electrophoresis. The buffer maintains the stability and integrity of the nucleic acids while they migrate through the gel matrix. The typical final concentration of TAE buffer used for gel electrophoresis is 1x or 0.5x, depending on the specific application. It is important to note that the optimal concentration may vary depending on factors such as the size of the nucleic acids being analyzed and the desired resolution.

Composition

Quick Direction

Dilute 50-fold to 1xTAE. In 50 ml 1xTAE, dissolve the following amount of GBP Agarose, Low EEO (CAT #28036) based on your intended range of separation or gel concentration.