Agarose, Low EEO
Agarose, Low EEO
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CAT No. #28036
Description
GBP Agarose, Low EEO (Low ElectroEndOsmosis) features ultra-high purity for enhanced gel electrophoresis resolution with shortened running time. Agarose is a linear polysaccharide derived from agar, a substance purified from seaweed. It consists of repeating units of agarobiose, a disaccharide composed of D-galactose and 3,6-anhydro-L-galactopyranose. Agarose has a gel-forming property, which allows it to form a network of interconnected pores when dissolved and cooled. This gel matrix is used in gel electrophoresis to separate biomolecules based on their size and charge. Agarose gels have excellent sieving properties and are commonly used in molecular biology for DNA, RNA, and protein analysis. Agarose is biocompatible and considered safe for use in biological applications.
Composition
Agarose, Low EEO (Molecular Biological Grade)
Technical Specification
|
Format |
White to off-white powder |
|
Gel strength |
≥1200 g/cm2 (1% Gel) |
|
Gelling temperature |
36±1.5℃ (1.5% Gel) |
|
Melting temperature |
88±1.5℃ (1.5% Gel) |
|
Electroosmotic value |
≤0.13-mr |
|
Sulfate |
≤0.15% |
|
Moisture |
≤10% |
|
DNase |
None Detected |
|
RNase |
None Detected |
|
Protease |
None Detected |
Quick Direction
For DNA gel electrophoresis, dissolve the desired amount of agarose powder based on your intended range of separation or gel concentration (Table 1).
Table 1. Separation Range for different agarose gel concentration.
|
Linear dsDNA Separation Range (bp) |
Concentration (%) |
Mass (g) to dissolve in 50ml, 1xTAE |
|
1,000 - 30,000 |
0.50 |
0.25 |
|
800 - 12,000 |
0.70 |
0.35 |
|
500 - 10,000 |
1.00 |
0.50 |
|
400 - 7,000 |
1.20 |
0.60 |
|
200 - 3,000 |
1.50 |
0.75 |
|
50 - 2,000 |
2.00 |
1.00 |
